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If only the substrate concentration is varied, it is not generally possible to fit both kcat and kM. However, it is possible to fit the ratio kcat/kM. Including this rstan::stan code will generate the samples for kcat/kM.

Usage

tQ_genquant()

References

Choi, B., Rempala, G.A. & Kim, J.K. Beyond the Michaelis-Menten equation: Accurate and efficient estimation of enzyme kinetic parameters. Sci Rep 7, 17018 (2017). https://doi.org/10.1038/s41598-017-17072-z

See also